Isopycnic-centrifugation studies in caesium chloride and in caesium sulphate on dermatan sulphate proteoglycans from bovine sclera
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منابع مشابه
Isolation and characterization of dermatan sulphate proteoglycans from bovine sclera.
1. Proteoglycans were extracted from sclera with 4 M-guanidine hydrochloride in the presence of proteinase inhibitors and purified by ion-exchange chromatography and density-gradient centrifugation. 2. The entire proteoglycan pool was characterized by compositional analyses and by specific chemical (periodate oxidation) and enzymic (chondroitinases) degradations. The glycan moieties of the mole...
متن کاملBovine aortic chondroitin sulphate- and dermatan sulphate-containing proteoglycans. Isolation, fractionation and chemical characterization.
1. Guanidinium chloride (4M) in the presence of proteinase inhibitors extracted 90% of bovine aorta galactosaminoglycans as proteoglycans that were subsequently purified by ion-exchange and gel chromatography. 2. Fractionation of the calcium salts of the purified proteoglycans with increasing concentration of ethanol yielded fractions PG-25 (28%), PG-35 (45%) and PG-50 (37%). 3. Fraction PG-50 ...
متن کاملStructure and properties of dermatan sulphate proteoglycans.
Introduction Fibrous connective tissues like sclera, skin, tendon and cornea have a high content of collagen but only small amounts of proteoglycans and structural glycoproteins. The specific blend of collagen and proteoglycan types determines the properties of the tissue. The proteoglycans are characterized by having a central protein core substituted by one or several glycosaminoglycan chains...
متن کاملDermatan sulphate proteoglycans from sclera examined by rotary shadowing and electron microscopy.
Two dermatan sulphate-containing proteoglycans from bovine sclera were examined by rotary shadowing and electron microscopy, and the results were compared with previous biochemical findings. Both the large iduronate-poor proteoglycan (PGI) and the small iduronate-rich proteoglycan (PGII) possessed a globular proteinaceous region. Whereas PGI had a branched extension from the globular region, wi...
متن کاملStructural variability of large and small chondroitin sulphate/dermatan sulphate proteoglycans.
Ledbetter, S. R., Tyree, B.. Hashell. J. R. & Horrigan. E. A. ( 1985) J . Biol. C'hrm. 260,8106-8 I 13 Hassell, J. R., Noonan, D. M., Ledbetter, S. R. & Laurie. G. W. (1986) C'ihri Found. Symp. 124,204-222 Noonan. D. M.. Horrigan. E. A.. Ledbetter. S. R.. Vogeli. G., Sasaki. M.. Yamada. Y. & Hassell. J . R. ( 1988) J . Riol. ('hem. 263, 16379-16387 Paulsson. M.. Yurchenko. P. D.. Ruben. G. C.. ...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1981
ISSN: 0264-6021
DOI: 10.1042/bj1990581